January 9, 2012 - Isolating DNA
Nikhat Zaidi, a biochemist for Biorad Laboratories, did a wonderful presentation on isolating DNA. The following two slides explains the beginning of what we did to isolate our own DNA!
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Thank you very much for coming to our classroom! I really enjoyed learning about DNA and how to isolate my own DNA. I like the fact that I have it in a necklace, and I can show it to other people! :)
ReplyDeleteThanks again!
Abigail Miller
I am jealous I didn't get to do this.
ReplyDeleteThank you Nikhat for coming to our class and showing us how to isolate our DNA. I loved showing the necklace to other people and actually seeing my DNA. It was so fascinating to learn the fact that I have the same DNA as my identical twin sister. I learned a lot and again thank you for coming to our class.
ReplyDeleteGil
I loved the project that we did! it was so fun and I got to show everyone my DNA necklace. I liked how the DNA was this white wisp. It looked so cool. How do they use DNA to clone people?
ReplyDeleteAndrew
Thank you so much for coming to our classroom! I really appreciate you coming over and showing us how to make our own DNA necklaces. I also have one question about DNA. Since it's like a blueprint for your body, how would you read it?
ReplyDeleteThank you!
Ange
Thanks so much! It was really fun to do the experiment and it was so cool how you could actually see your DNA. I never imagined it to look like that. I have always thought of it like this: http://www.msg.ucsf.edu/local/programs/ribbons/help/dna_rgb.gif
ReplyDeleteThanks Again!
Susannah
Thank you very much for coming into our classroom and teaching us about our DNA and your job. I was really interested in my DNA and how I could see it and you job sounds awesome! Also the necklace idea was great! I could show my friends and family my DNA without any problem. Thank you very much for taking the time to make that slideshow and come to our class! I learned a lot about DNA and what a biochemist does. Thanks again!
ReplyDeleteNoy
Thank you so much for coming into our class and teaching us how to visualize our own DNA using that cool kit! I think that it is really interesting that the DNA is in a necklace.
ReplyDeleteSincerely,
Ella
Thank you for coming to our class! It was probably the coolest science experiment I've ever done!I never really knew what DNA was exactly, or what it looked like. I never knew you could make a necklace out of it! I really learned a lot. Thanks again!
ReplyDelete--Elizabeth
Thank you for coming to our classroom! I really enjoyed isolating my DNA and putting it into a necklace!
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Elaine
Now I know more about DNA and how to isolate it. I really enjoyed learning about DNA.
ReplyDeleteThank You!
Benji
I really liked you DNA experiment. What I mostly like was the DNA samples. I thought that looking at your DNA was impossible without a microscope!
ReplyDeleteThank you so much for teaching us how to isolate our DNA! I learned that only 1% of someone's DNA is different from another person's. I enjoyed doing this activity and learning new things about DNA. Thanks again!
ReplyDeleteSincerely,
Yordi Dawit
wow so cool.
ReplyDeleteThank you for taking time off from work to come to our classroom! I learned so many things about DNA that I never knew before. I was really surprised when you said that 99.9% of DNA in all human beings is the same. That means 0.1% of our DNA makes us different. I really enjoyed isolating my DNA and putting it in a pendant that I can wear around my neck.
ReplyDeleteThank you again!
Maya
Thank you so much, Mrs.Zaidi, for that lesson you did on DNA, it was very interesting. I learned how only 1% of all people's DNA is different from another person's DNA. I thought before that everyone is 100% different. I didn't know you could see DNA, until you showed us, I thought before you could only see DNA with a microscope. Thanks for the fascinating information!
ReplyDeleteThank you so much mrs. Zaidi! That was so much fun! I lovers your presentation. It was both fun and interesting at the same time! I love wearing my DNA around my neck!i could not believe that only 1% of our DNA was different from the others! I also loved your presentation! I liked chewing my cheek cells off also! I think they grew back! One question in would like to ask you is how are our parents dnas get combined? Do they travel?
ReplyDeleteThank you so much again!
Sincerely
Jenny lipof
I really liked you DNA experiment. What I mostly like was the DNA samples. I thought that looking at your DNA was impossible without a microscope!
ReplyDeleteThank you
Adir
Thank you so much i thought it was really cool they we could get our DNA by just swishing water in our mouth.
ReplyDeleteJack B
thank you mrs.Zaidi,for coming into our classroom and teaching us about how to isolating our DNA it was really cool
ReplyDeletethank you again
Tate
Thank you, Mrs.Zaidi,
ReplyDeletefor coming to our class and teaching us about DNA! I never thought I'd be able to see my DNA without a microscope! I also worn my DNA necklace around the whole day, and everyone on the other teams though it was really cool!
Thanks again,
Emma
Nikhat,
ReplyDeleteIt was so wonderful having you visit us in our classroom and you teaching us a part of the big world about DNA! I never knew that 99% of us is the same as everyone else, and only 1% of us makes us different! If your an identical twin, would it be 100% for you and your twin?
Thank you so much!
Isabela P
Nikhat, Thank you so much for coming in on your on time to teach us something new it was so cool to take our DNA i've never done that before.
DeleteThank you so much.
Olyvia
It was very nice to have you visit our classroom and I really enjoyed seeing my DNA. Do you also work with analyzing DNA patterns?
DeleteJack Sinclair
Thank you so much for coming in to our class and teaching us about biochemistry. I had a lot of fun finding my own DNA. I learned so many things from your presentation. I learned that Only 1% of every human is different. I thought that that was crazy! Thank you again for coming to our class!
ReplyDeleteJordan
Thanks,
ReplyDeleteFor coming into our class! It was so fun and my DNA looks so cool. Now I can have a little piece of me around my neck from time to time. It very educational and fastanating. Thanks again.
,Lindsay C
Thank you all for having me over to do the STEM career presentation and the DNA isolation experiment with you. I am glad that you enjoyed the experiment, I did have a lot of fun spending time with you and teaching you about DNA. Special thanks go to Ms. Sullivan and Mr. Bunting for rearranging their class schedule to accommodate us when our experiment ran overtime, also to Mrs. MacNeil for helping out with our experiment.
ReplyDeleteI know that you all were very surprised to see your DNA with naked eye without a microscope. Strands of DNA are colorless and so thin that it is not possible to see them when they are in solution. In your experiment when you added cold alcohol, it precipitated them, all those strands come together and form this wispy mass that you were able to see. Here is what your wispy DNA mass would have looked like if we looked it under a very powerful electron microscope.
UNABLE TO DOWNLOAD PICTURE.
McGready J. Cell Sci. 39, 53-62 (1979)
Here are the answers to your questions:
Ange: As you know that DNA encodes all the information that is needed to make a human being or any other living organism. It is made of 4 basic repeating units called "Nucleotides". Each cell in your body contains about 3 billion nucleotides and this whole nucleotide sequence is the Blueprint and called a Genome. Recently, scientists have sequenced or in other words have read the human genome. You need a special lab technique known as DNA sequencing to tell in which order these nucleotides are strung together in your DNA.
Andrew: The word cloning means making an exact copy of something. In pop culture, human cloning is often referred as creating another exact copy of a human being. However, no real real humans have been cloned yet, only human clones that exist in nature are "IDENTICAL TWINS". Although, animals have been cloned, first animal cloned was a sheep named Dolly. this gives people notion that some day humans will be cloned. There are ethical issue associated with human cloning therefore human cloning is banned/controversial in most countries. Cloning a whole human being should not confused with human therapeutic cloning practice that could provide genetically identical cells for regenerative medicine, and tissues and organs for transplantation. Such cells can be used to treat serious diseases such as cancer, heart disease and diabetes, as well as improvements in burn treatment and reconstructive and cosmetic surgery.
Isabela: Yes, identical twins will have the same DNA. Although scientists have shown that if the identical twins are exposed to different environments (something that can cause their DNA to mutate) they may have a few minor differences in their DNA.
Jack S: I assume you are asking about DNA pattern analysis with regards to Forensics. To identify individuals, forensic scientists scan 13 DNA regions, or loci that vary from person to person and use the data to create a DNA profile of that individual (sometimes called a DNA fingerprint). There is an extremely small chance that another person has the same DNA profile for a particular set of 13 regions.
I do not do DNA pattern analysis in that sense but I have cut DNA using various restriction enzymes also known as molecular scissors.
Jenny L: You are right, your cheeks cells have grown back. They are just like the skin cells they are constantly dividing (growing) and sloughing” off (shedding).
Susannah K: Ribbon like double helix structure that you see in the pictures is the schematic representation of what DNA would like based upon its chemcial structure.
Celine Y: Thank you for taking to time to come and teach us about DNA. I had fun learning about DNA and isolating it {especially into a necklace!} Thank you!
DeleteThank you so much, Mrs.Zaidi, for that lesson you did on DNA, it was very interesting. I learned how only 1% of all people's DNA is different from another person's DNA. I thought before that everyone is 100% different. I didn't know you could see DNA, until you showed us, I thought before you could only see DNA with a microscope. Thanks for the fascinating information!
ReplyDeleteKaren
Thank you so much for coming in and talking to us! I had alot of fun learning about DNA and especially love being to see it!
ReplyDelete-Isabel
thank you for takeing time from your schedule to come and teach us how to make dna keven
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